Papaya Papain

The papain is reaped from the carica papaya fruit. It’s a pure natural, healthy, safe and high-effective enzyme preparation after bio-technology’s extract, microfiltration, ultrafiltration and freeze-drying. This product is a proteinase compound includes papain, rennase and peptidase. It has strong catalyzed activity of the protein hydrolysis, curd and lysis activity. It also owns the lipolysis and protein synthesis ability. The function is mainly catalyzing the hydrolysis of protein, there are many cutting point, prior to decompose the peptide link of arginine and PHE. This product is compose by 212 amino acids, the molecular weight is 21000, belong to a peptide chain incision enzyme that include –SH. Due to papain’s high activity and heat stability, it can widely apply to food, pharmaceutical, daily-chemical, feed, leather and textile industry etc.

ENZYMOLYSIS PRINCIPLE

In certain temperature, pH value and substrate concentration, papain can hydrolyze protein to peptone, polypeptine and amino acid etc.

Activity definition

UV Spectrophotometry： in test condition(37±0.2℃； PH value 7.0). every minute hydrolyze casein and release soluble TDA, when the TDA absorbance is quite with 1 microgram tyrosine in 275nm, the needed enzyme is a unit, express by u/g.

Properties of Papain:

• Enzyme Type: It is a cysteine protease, belonging to the peptidase C1 family.
• Source: It is isolated from papaya (Carica papaya L.) latex.
• Activity: The activity of papain is directly related to the ripeness of the fruit, with greener fruits having higher activity.
• Appearance: It is typically obtained as a lyophilized (freeze-dried) powder, almost white or sometimes pale yellow crystal powder.
• Solubility: It is soluble in water but insoluble in most organic solvents.
• Stability:  Exhibits good thermal stability.
• Safety: It is considered safe for various applications.

Uses of Papain:

1. Food Industry:

• It is used as a food additive, especially in meat tenderization, where it breaks down tough proteins to make meat more tender.
• It is also used in brewing and baking industries to improve protein breakdown and enhance flavor.

2. Pharmaceutical Industry:

• In pharmaceuticals, papain finds applications in digestive aids, wound healing ointments, and anti-inflammatory drugs.
• It’s used in the formulation of various medications due to its proteolytic properties.

3. Textile Industry:

• It is utilized in the textile industry for fabric softening and in the removal of hair from hides during leather processing.

4. Cosmetic Industry:

• Due to its exfoliating properties, papain is used in skincare products, such as facial scrubs and masks, to remove dead skin cells and improve skin texture.

5. Other Industries:

• It is also used in the production of animal feed to improve digestibility.
• It has applications in the production of detergents and DNA extraction processes due to its protein-dissolving capabilities.

Packaging and Storage:

• It is typically packaged in carton boxes or drums.
• It should be stored in a cool environment between 2-8°C to maintain its stability.
• The shelf life of papain is typically greater than 12 months when stored under appropriate conditions.

Safety Considerations:

• While papain is generally considered safe, individuals with allergies to latex or papaya should avoid products containing papain.
• Proper handling and storage procedures should be followed to prevent contamination and maintain enzyme activity.

Overall, papain is a valuable enzyme with diverse applications across multiple industries, owing to its proteolytic activity and natural origin.

Product Sheet